Analysis Note

Positive ControlHepG2 cells

Negative Control293T cells

Application

ELISA (see coments)r>Immunoblotting (1-5 µg/ml)r>Immunocytochemistry (10 µg/ml)

General description

Purified mouse monoclonal antibody. Recognizes the ~70-75 kDa AFP protein.

Recognizes the ~70-75 kDa AFP protein in HepG2 cells.

Anti-AFP, mouse monoclonal, clone 1G7, recognizes ~70-75 kDa AFP protein in HepG2 cells. It is validated for ELISA, Western blotting, and immunocytochemistry.

Immunogen

A recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

100 µg in Glass bottle

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)